Corneal and Ocular Immunology Unit

Researcher

Research Overview

Overview

Our research is aimed at understanding how corneal epithelial cells, immune cells and sensory nerves interact to achieve homeostasis and rapid recovery following injury, exposure to noxious stimuli and during normal ageing. Our laboratory is based in the Melbourne Brain Centre, where we have access to a suite of state-of-the-art instruments to enable in vivo clinical imaging of the mouse eye, along with ex vivo imaging of corneas, tissue culture and molecular biology techniques.

Corneal immune cells

The mouse and human cornea contains populations of resident immune cells such as macrophages and dendritic cells. These cells play an important role in generating innate inflammatory responses against microbial pathogens and sterile injurious stimuli, as well as interacting with the dense population of sensory nerves that supply the corneal epithelium.

Neuroimmune interactions

Recent studies suggest that corneal dendritic cells are closely associated with corneal sensory nerves. The nature of this relationship is thought to be spatial (i.e. the two entities are physically in contact), physiological (dendritic cells are thought to promote nerve homeostasis) and immunological (i.e. nerve stimulation affects dendritic cell activation). We have a range of active projects in the lab investigating the interplay of corneal sensory nerves and the effect that tissue injury and exposure to noxious stimuli has on the activation state of the resident immune cells.

Major techniques

  • In vivo clinical imaging of corneal inflammation using spectral domain optical coherence tomography
  • Ex vivo confocal imaging of immunostained tissues, 3D reconstruction and image analysis
  • Analysis of chemokine, cytokine and neuropeptide gene expression and production (qPCR and ELISA

Other research interests

Staff

Helen Jiao (Post-doctoral fellow)

Students

Manikkuwadura De Silva (PhD student)

Kirthana Senthil (Master of Biomedical Science student)

Mengliang Wu (PhD student)

Collaborators

  • Dr Laura Downie, Department of Optometry and Vision Sciences, University of Melbourne
  • Dr Lisa Hill, Institute of Inflammation and Ageing, University of Birmingham
  • Prof Paul McMenamin, Department of Anatomy and Developmental Biology, Monash University
  • Dr Samantha Dando, Department of Anatomy and Developmental Biology, Monash University

Funding

This lab has been funded by the NH&MRC since 2013.

Research Outcomes

Corneal macrophages (red) located beneath the epithelial nerve plexus (gray) in the central mouse cornea.

A false coloured depth projection of the epithelial nerve plexus in the central cornea of a mouse.

Iba-1+CD45+ dendritic cells (yellow) interacting with CD45+ myeloid cells in the epithelium of the mouse cornea.

A cross-sectional view of the mouse anterior segment imaged using spectral domain optical coherence tomography.

Research Projects

This Research Group doesn't currently have any projects



Faculty Research Themes

Infection and Immunology

School Research Themes

Immunology and Inflammation



Key Contact

For further information about this research, please contact Dr Holly Chinnery

Department / Centre

Optometry and Vision Sciences

Unit / Centre

Corneal and Ocular Immunology Unit


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